Plasmid transformation of Streptococcus lactis protoplasts: optimization and use in molecular cloning.

The parameters affecting polyethylene glycol-induced plasmid transformation of Streptococcus lactis LM0230 protoplasts were examined to increase the transformation frequency. In contrast to spreading protoplasts over the surface of an agar medium, their incorporation into soft agar overlays enhanced...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Kondo, J K, McKay, L L
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1984
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC241498/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6091544/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.48.2.252-259.1984
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