Plasmid transformation of Streptococcus lactis protoplasts: optimization and use in molecular cloning.
The parameters affecting polyethylene glycol-induced plasmid transformation of Streptococcus lactis LM0230 protoplasts were examined to increase the transformation frequency. In contrast to spreading protoplasts over the surface of an agar medium, their incorporation into soft agar overlays enhanced...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1984
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC241498/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6091544/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.48.2.252-259.1984 |
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