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LC-MS/MS Analysis of Apical and Basolateral Plasma Membranes of Rat Renal Collecting Duct Cells

We used biotinylation and streptavidin affinity chromatography to label and enrich proteins from apical and basolateral membranes of rat kidney inner medullary collecting ducts (IMCDs) prior to LC-MS/MS protein identification. To enrich apical membrane proteins and bound peripheral membrane proteins...

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Bibliografiske detaljer
Main Authors: Yu, Ming-Jiun, Pisitkun, Trairak, Wang, Guanghui, Shen, Rong-Fong, Knepper, Mark A.
Format: Artigo
Sprog:Inglês
Udgivet: 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2412072/
https://ncbi.nlm.nih.gov/pubmed/16899541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M600177-MCP200
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