LC-MS/MS Analysis of Apical and Basolateral Plasma Membranes of Rat Renal Collecting Duct Cells

We used biotinylation and streptavidin affinity chromatography to label and enrich proteins from apical and basolateral membranes of rat kidney inner medullary collecting ducts (IMCDs) prior to LC-MS/MS protein identification. To enrich apical membrane proteins and bound peripheral membrane proteins...

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Hlavní autoři: Yu, Ming-Jiun, Pisitkun, Trairak, Wang, Guanghui, Shen, Rong-Fong, Knepper, Mark A.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2006
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2412072/
https://ncbi.nlm.nih.gov/pubmed/16899541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M600177-MCP200
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