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PCR-based gene synthesis to produce recombinant proteins for crystallization
BACKGROUND: Gene synthesis technologies are an important tool for structural biology projects, allowing increased protein expression through codon optimization and facilitating sequence alterations. Existing methods, however, can be complex and not always reproducible, prompting researchers to use c...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2408586/ https://ncbi.nlm.nih.gov/pubmed/18445293 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-8-44 |
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