Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.

A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking PCR primers to generate a 607 base pair gene which contained 31 unique locations for restriction enzyme...

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Podrobná bibliografie

Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Graham, R W, Atkinson, T, Kilburn, D G, Miller, R C, Warren, R A
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 1993
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311407/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8177740/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.21.4923
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