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Identification of soluble protein fragments by gene fragmentation and genetic selection

We describe a new method, which identifies protein fragments for soluble expression in Escherichia coli from a randomly fragmented gene library. Inhibition of E. coli dihydrofolate reductase (DHFR) by trimethoprim (TMP) prevents growth, but this can be relieved by murine DHFR (mDHFR). Bacterial stra...

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Autors principals: Dyson, Michael R., Perera, Rajika L., Shadbolt, S. Paul, Biderman, Lynn, Bromek, Krystyna, Murzina, Natalia V., McCafferty, John
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2008
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2396403/
https://ncbi.nlm.nih.gov/pubmed/18420658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn151
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