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A FRET based mechanical stress sensor for specific proteins in situ
To measure mechanical stress in real time we designed a fluorescence energy transfer (FRET) cassette, noted stFRET, which could be inserted into structural protein hosts. The probe was made of a green fluorescence protein (GFP) pair, Cerulean and Venus, linked with a stable α-helix. We measured the...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2396198/ https://ncbi.nlm.nih.gov/pubmed/18479457 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1742-4658.2008.06461.x |
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