Inability of Real-Time Reverse Transcriptase PCR Assay To Detect Subtype H7 Avian Influenza Viruses Isolated from Wild Birds

We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed fo...

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Hlavní autoři: Xing, Zheng, Cardona, Carol, Dao, Phuong, Crossley, Beate, Hietala, Sharon, Boyce, Walter
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2008
Témata:
Clinical Veterinary Microbiology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2395069/
https://ncbi.nlm.nih.gov/pubmed/18322070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02426-07
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