Inability of Real-Time Reverse Transcriptase PCR Assay To Detect Subtype H7 Avian Influenza Viruses Isolated from Wild Birds

We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed fo...

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Autores principales: Xing, Zheng, Cardona, Carol, Dao, Phuong, Crossley, Beate, Hietala, Sharon, Boyce, Walter
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 2008
Materias:
Clinical Veterinary Microbiology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2395069/
https://ncbi.nlm.nih.gov/pubmed/18322070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02426-07
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