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Insight from TonB Hybrid Proteins into the Mechanism of Iron Transport through the Outer Membrane

We created hybrid proteins to study the functions of TonB. We first fused the portion of Escherichia coli tonB that encodes the C-terminal 69 amino acids (amino acids 170 to 239) of TonB downstream from E. coli malE (MalE-TonB69C). Production of MalE-TonB69C in tonB(+) bacteria inhibited siderophore...

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Detalhes bibliográficos
Main Authors: Kaserer, Wallace A., Jiang, Xiaoxu, Xiao, Qiaobin, Scott, Daniel C., Bauler, Matthew, Copeland, Daniel, Newton, Salete M. C., Klebba, Phillip E.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2395051/
https://ncbi.nlm.nih.gov/pubmed/18390658
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00135-08
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