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Fluoresceination of FepA during Colicin B Killing: Effects of Temperature, Toxin and TonB
We studied the reactivity of 35 genetically engineered Cys sulfhydryl groups at different locations in Escherichia coli FepA. Modification of surface loop residues by fluorescein maleimide (FM) was strongly temperature-dependent in vivo, whereas reactivity at other sites was much less affected. Cont...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3082853/ https://ncbi.nlm.nih.gov/pubmed/19432807 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2958.2009.06715.x |
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