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Fluoresceination of FepA during Colicin B Killing: Effects of Temperature, Toxin and TonB
We studied the reactivity of 35 genetically engineered Cys sulfhydryl groups at different locations in Escherichia coli FepA. Modification of surface loop residues by fluorescein maleimide (FM) was strongly temperature-dependent in vivo, whereas reactivity at other sites was much less affected. Cont...
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| 主要な著者: | , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2009
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3082853/ https://ncbi.nlm.nih.gov/pubmed/19432807 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2958.2009.06715.x |
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