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Analysis of Bacterial Communities in Soil by Use of Denaturing Gradient Gel Electrophoresis and Clone Libraries, as Influenced by Different Reverse Primers

To assess soil bacterial diversity, PCR systems consisting of several slightly different reverse primers together with forward primer F968-GC were used along with subsequent denaturing gradient gel electrophoresis (DGGE) or clone library analyses. In this study, a set of 13 previously used and novel...

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Détails bibliographiques
Auteurs principaux: Brons, Jolanda K., van Elsas, Jan Dirk
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2008
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394888/
https://ncbi.nlm.nih.gov/pubmed/18310425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02195-07
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