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Analysis of Bacterial Communities in Soil by Use of Denaturing Gradient Gel Electrophoresis and Clone Libraries, as Influenced by Different Reverse Primers

To assess soil bacterial diversity, PCR systems consisting of several slightly different reverse primers together with forward primer F968-GC were used along with subsequent denaturing gradient gel electrophoresis (DGGE) or clone library analyses. In this study, a set of 13 previously used and novel...

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Detalhes bibliográficos
Main Authors: Brons, Jolanda K., van Elsas, Jan Dirk
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2394888/
https://ncbi.nlm.nih.gov/pubmed/18310425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02195-07
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