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Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters

Genetically encoded sensors of glutamate concentration are based on FRET between cyan and yellow fluorescent proteins bracketing a bacterial glutamate-binding protein. Such sensors have yet to find quantitative applications in neurons, because of poor response amplitude in physiological buffers or w...

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Detalhes bibliográficos
Main Authors: Hires, Samuel Andrew, Zhu, Yongling, Tsien, Roger Y.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2393813/
https://ncbi.nlm.nih.gov/pubmed/18332427
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0712008105
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