Rapid extraction of plasmids from Clostridium perfringens.

Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and e...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Main Authors: Mahony, D E, Clark, G A, Stringer, M F, MacDonald, M C, Duchesne, D R, Mader, J A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1986
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238912/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2870680/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.51.3.521-523.1986
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