Rapid extraction of plasmids from Clostridium perfringens.

Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and e...

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Dettagli Bibliografici
Autori principali: Mahony, D E, Clark, G A, Stringer, M F, MacDonald, M C, Duchesne, D R, Mader, J A
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1986
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC238912/
https://ncbi.nlm.nih.gov/pubmed/2870680
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