Rapid extraction of plasmids from Clostridium perfringens.
Two rapid methods were evaluated for their extraction of plasmids from Clostridium perfringens. The first method involved lysis of 1 to 2 ml of C. perfringens culture by treatment with hyaluronidase, lysozyme, and sarcosyl. DNA, extracted with phenol-chloroform, was treated with RNase, boiled, and e...
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| Publicat a: | Appl Environ Microbiol |
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| Autors principals: | , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1986
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238912/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2870680/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.51.3.521-523.1986 |
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