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Rapid physical mapping by transposon Tn5 mutagenesis to localize the cloned yeast ILV2 gene.

A rapid method for Tn5 mutagenesis of cloned genes on multicopy plasmids was used to map a yeast ILV2 mutant allele encoding a sulfometuron methyl-resistant acetolactate synthase. Twenty-one of 40 independent Tn5 insertions were within the 5.6-kilobase-pair cloned segment. Of these, seven adjacent t...

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Autores principales: Van Dyk, T K, Falco, S C, LaRossa, R A
Formato: Artigo
Lenguaje:Inglês
Publicado: 1986
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC238841/
https://ncbi.nlm.nih.gov/pubmed/3006586
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