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Rapid physical mapping by transposon Tn5 mutagenesis to localize the cloned yeast ILV2 gene.
A rapid method for Tn5 mutagenesis of cloned genes on multicopy plasmids was used to map a yeast ILV2 mutant allele encoding a sulfometuron methyl-resistant acetolactate synthase. Twenty-one of 40 independent Tn5 insertions were within the 5.6-kilobase-pair cloned segment. Of these, seven adjacent t...
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| Publicado en: | Appl Environ Microbiol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1986
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238841/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3006586/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.51.1.206-208.1986 |
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