Rapid physical mapping by transposon Tn5 mutagenesis to localize the cloned yeast ILV2 gene.

A rapid method for Tn5 mutagenesis of cloned genes on multicopy plasmids was used to map a yeast ILV2 mutant allele encoding a sulfometuron methyl-resistant acetolactate synthase. Twenty-one of 40 independent Tn5 insertions were within the 5.6-kilobase-pair cloned segment. Of these, seven adjacent t...

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Publicat a:Appl Environ Microbiol
Autors principals: Van Dyk, T K, Falco, S C, LaRossa, R A
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1986
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238841/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3006586/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.51.1.206-208.1986
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