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Cloning of the pullulanase gene and overproduction of pullulanase in Escherichia coli and Klebsiella aerogenes.

The pullulanase gene (pul) of Klebsiella aerogenes was cloned into a pBR322 vector in Escherichia coli. Deletion analysis of the recombinant plasmid showed that the pul coding sequence, probably with the regulator gene, was located entirely within a 4.2-kilobase segment derived from the chromosomal...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Takizawa, N, Murooka, Y
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238396/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3885852/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.49.2.294-298.1985
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