Cloning of the pullulanase gene and overproduction of pullulanase in Escherichia coli and Klebsiella aerogenes.
The pullulanase gene (pul) of Klebsiella aerogenes was cloned into a pBR322 vector in Escherichia coli. Deletion analysis of the recombinant plasmid showed that the pul coding sequence, probably with the regulator gene, was located entirely within a 4.2-kilobase segment derived from the chromosomal...
Shranjeno v:
| izdano v: | Appl Environ Microbiol |
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| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1985
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238396/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3885852/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.49.2.294-298.1985 |
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