In vitro expression of the human cytomegalovirus DNA polymerase gene: effects of sequence alterations on enzyme activity.
Genomic DNA of the Towne strain human cytomegalovirus polymerase (pol) gene (4.4-kb RsrII-NcoI segment of the EcoRI J fragment) was cloned into plasmids containing either the T3 or the T7 promoter for in vitro transcription-translation studies. The translation efficiency of unmodified pol cRNA was p...
Enregistré dans:
| Publié dans: | J Virol |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1993
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238068/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8411337/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.67.11.6339-6347.1993 |
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