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In vitro expression of the human cytomegalovirus DNA polymerase gene: effects of sequence alterations on enzyme activity.

Genomic DNA of the Towne strain human cytomegalovirus polymerase (pol) gene (4.4-kb RsrII-NcoI segment of the EcoRI J fragment) was cloned into plasmids containing either the T3 or the T7 promoter for in vitro transcription-translation studies. The translation efficiency of unmodified pol cRNA was p...

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Detalhes bibliográficos
Publicado no:J Virol
Principais autores: Ye, L B, Huang, E S
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC238068/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8411337/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.67.11.6339-6347.1993
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