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Crystallization and preliminary X-ray characterization of the catalytic domain of collagenase G from Clostridium histolyticum

The catalytic domain of collagenase G from Clostridium histolyticum has been cloned, recombinantly expressed in Escherichia coli and purified using affinity and size-exclusion column-chromatographic methods. Crystals of the catalytic domain were obtained from 0.12 M sodium citrate and 23%(v/v) PEG 3...

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Detalhes bibliográficos
Main Authors: Eckhard, Ulrich, Nüss, Dorota, Ducka, Paulina, Schönauer, Esther, Brandstetter, Hans
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2376405/
https://ncbi.nlm.nih.gov/pubmed/18453715
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309108010476
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