Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of variants of monoamine oxidase from Aspergillus niger

Monoamine oxidase from Aspergillus niger (MAO-N) is an FAD-dependent enzyme that catalyses the conversion of terminal amines to their corresponding aldehydes. Variants of MAO-N produced by directed evolution have been shown to possess altered substrate specificity. Crystals of two of these variants...

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Bibliographic Details
Main Authors: Atkin, Kate E., Reiss, Renate, Turner, Nicholas J., Brzozowski, Andrzej M., Grogan, Gideon
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2008
Subjects:
Crystallization Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374162/
https://ncbi.nlm.nih.gov/pubmed/18323603
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910800345X
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