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Overproduction, purification, crystallization and preliminary X-ray analysis of the peroxiredoxin domain of a larger natural hybrid protein from Thermotoga maritima

Thermotoga maritima contains a natural hybrid protein constituted of two moieties: a peroxiredoxin domain at the N-terminus and a nitroreductase domain at the C-terminus. The peroxiredoxin (Prx) domain has been overproduced and purified from Escherichia coli cells. The recombinant Prx domain, which...

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Detalhes bibliográficos
Main Authors: Barbey, Carole, Rouhier, Nicolas, Haouz, Ahmed, Navaza, Alda, Jacquot, Jean-Pierre
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374002/
https://ncbi.nlm.nih.gov/pubmed/18097097
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107064391
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