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Semi-automated microseeding of nanolitre crystallization experiments

A simple semi-automated microseeding procedure for nanolitre crystallization experiments is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon bead. A dilution series of this microseed stock is then prepared and dispensed as 100 nl droplets into 96-well crystall...

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Detalhes bibliográficos
Main Authors: Walter, Thomas S., Mancini, Erika J., Kadlec, Jan, Graham, Stephen C., Assenberg, René, Ren, Jingshan, Sainsbury, Sarah, Owens, Raymond J., Stuart, David I., Grimes, Jonathan M., Harlos, Karl
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373990/
https://ncbi.nlm.nih.gov/pubmed/18097093
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107057260
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