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Effects of i-propanol on the structural dynamics of Thermomyces lanuginosa lipase revealed by tryptophan fluorescence

Influence of isopropanol (iPrOH) on the structural dynamics of Thermomyces lanuginosa lipase (TLL) was studied by steady-state, time-resolved, and stopped-flow fluorescence spectroscopy, monitoring the intrinsic emission of Trp residues. The fluorescence of the four Trps of the wild-type enzyme repo...

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Autors principals: Zhu, Keng, Jutila, Arimatti, Tuominen, Esa K.J., Kinnunen, Paavo K.J.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373954/
https://ncbi.nlm.nih.gov/pubmed/11266620
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