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Effects of i-propanol on the structural dynamics of Thermomyces lanuginosa lipase revealed by tryptophan fluorescence
Influence of isopropanol (iPrOH) on the structural dynamics of Thermomyces lanuginosa lipase (TLL) was studied by steady-state, time-resolved, and stopped-flow fluorescence spectroscopy, monitoring the intrinsic emission of Trp residues. The fluorescence of the four Trps of the wild-type enzyme repo...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Cold Spring Harbor Laboratory Press
2001
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2373954/ https://ncbi.nlm.nih.gov/pubmed/11266620 |
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