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The distinctive functions of the two structural calcium atoms in bovine pancreatic deoxyribonuclease

The two amino acid residues, Asp 99 and Asp 201, involved in the coordination of the two calcium atoms in the X-ray structure of bovine pancreatic (bp) DNase, were individually changed by site-directed mutagenesis. The two altered proteins, brDNase(D99A) and brDNase(D201A) were expressed in Escheric...

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Detalles Bibliográficos
Main Authors: Chen, Ching-Ying, Lu, Shao-Chun, Liao, Ta-Hsiu
Formato: Artigo
Idioma:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2002
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2373464/
https://ncbi.nlm.nih.gov/pubmed/11847288
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