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Structure determinants of substrate specificity of hydroxynitrile lyase from Manihot esculenta

Tryptophan 128 of hydroxynitrile lyase of Manihot esculenta (MeHNL) covers a significant part of a hydrophobic channel that gives access to the active site of the enzyme. This residue was therefore substituted in the mutant MeHNL-W128A by alanine to study its importance for the substrate specificity...

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Detalhes bibliográficos
Main Authors: Lauble, Hanspeter, Miehlich, Burkhard, Förster, Siegfried, Kobler, Christoph, Wajant, Harald, Effenberger, Franz
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2002
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2368774/
https://ncbi.nlm.nih.gov/pubmed/11742123
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