Identification of active sites in amidase: Evolutionary relationship between amide bond- and peptide bond-cleaving enzymes

Mainly based on various inhibitor studies previously performed, amidases came to be regarded as sulfhydryl enzymes. Not completely satisfied with this generally accepted interpretation, we performed a series of site-directed mutagenesis studies on one particular amidase of Rhodococcus rhodochrous J1...

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Bibliographic Details
Main Authors: Kobayashi, Michihiko, Fujiwara, Yoshie, Goda, Masahiko, Komeda, Hidenobu, Shimizu, Sakayu
Format: Artigo
Language:Inglês
Published: The National Academy of Sciences of the USA 1997
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC23678/
https://ncbi.nlm.nih.gov/pubmed/9342349
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