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Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures.

After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies. IMAGES:

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Detalhes bibliográficos
Publicado no:J Bacteriol
Main Authors: Cabib, E, Duran, A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1975
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC236077/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/372/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.124.3.1604-1606.1975
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