Loading...
Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures.
After mutagenesis, surviving yeast cells are grown on plates at 25 C and later exposed to 37 C. The plates are then overlaid with a soft agar containing p-nitrophenylphosphate at pH 9.7. Lysed cells liberate alkaline phosphatase which gives rise to a yellow color on and around colonies.
Na minha lista:
| Main Authors: | , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1975
|
| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC236077/ https://ncbi.nlm.nih.gov/pubmed/372 |
| Tags: |
Tilføj Tag
Ingen Tags, Vær først til at tagge denne postø!
|