Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.

A method that gives high recovery of deoxyribonucleic acid (DNA) from crude bacterial lysates using ethidium bromide-cesium chloride density gradient centrifugation is presented. After Pronase digestion and shearing of the lysate, essentially 100% recovery of chromosomal DNA and a reproducible recov...

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Библиографические подробности

Опубликовано в::	J Bacteriol
Главные авторы:	Womble, D D, Taylor, D P, Rownd, R H
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 1977
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC235186/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/323223/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.130.1.148-153.1977
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