Method for obtaining more-accurate covalently closed circular plasmid-to-chromosome ratios from bacterial lysates by dye-buoyant density centrifugation.

A method that gives high recovery of deoxyribonucleic acid (DNA) from crude bacterial lysates using ethidium bromide-cesium chloride density gradient centrifugation is presented. After Pronase digestion and shearing of the lysate, essentially 100% recovery of chromosomal DNA and a reproducible recov...

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Bibliografske podrobnosti
Main Authors: Womble, D D, Taylor, D P, Rownd, R H
Format: Artigo
Jezik:Inglês
Izdano: 1977
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC235186/
https://ncbi.nlm.nih.gov/pubmed/323223
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