Isolation and properties of a thermostable restriction endonuclease (ENDO R-Bst1503).

A restriction endonuclease was isolated from Bacillus stearothermophilus1503-4R (Bst1503) and purified to homogeneity. The enzyme required Mg2+ ion as a cofactor. Bst1503 exhibited maximal activity between pH 7.5 and 8.0, between 60 and 65 degrees C, and with about 0.2 mM Mg2+. Bst1503 was not inact...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Catterall, J F, Welker, N E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1977
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC235052/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14105/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.129.2.1110-1120.1977
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