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Isolation and properties of a thermostable restriction endonuclease (ENDO R-Bst1503).

A restriction endonuclease was isolated from Bacillus stearothermophilus1503-4R (Bst1503) and purified to homogeneity. The enzyme required Mg2+ ion as a cofactor. Bst1503 exhibited maximal activity between pH 7.5 and 8.0, between 60 and 65 degrees C, and with about 0.2 mM Mg2+. Bst1503 was not inact...

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Bibliografiske detaljer
Main Authors: Catterall, J F, Welker, N E
Format: Artigo
Sprog:Inglês
Udgivet: 1977
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC235052/
https://ncbi.nlm.nih.gov/pubmed/14105
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