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Isolation and properties of a thermostable restriction endonuclease (ENDO R-Bst1503).

A restriction endonuclease was isolated from Bacillus stearothermophilus1503-4R (Bst1503) and purified to homogeneity. The enzyme required Mg2+ ion as a cofactor. Bst1503 exhibited maximal activity between pH 7.5 and 8.0, between 60 and 65 degrees C, and with about 0.2 mM Mg2+. Bst1503 was not inact...

詳細記述

保存先:
書誌詳細
主要な著者: Catterall, J F, Welker, N E
フォーマット: Artigo
言語:Inglês
出版事項: 1977
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC235052/
https://ncbi.nlm.nih.gov/pubmed/14105
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