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The complex between SOS3 and SOS2 regulatory domain from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis

The salt-tolerance genes SOS3 (salt overly sensitive 3) and SOS2 (salt overly sensitive 2) regulatory domain of Arabidopsis thaliana were cloned into a polycistronic plasmid and the protein complex was expressed in Escherichia coli, allowing purification to homogeneity in three chromatographic steps...

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Detalhes bibliográficos
Main Authors: Sánchez-Barrena, María José, Moreno-Pérez, Sandra, Angulo, Iván, Martínez-Ripoll, Martín, Albert, Armando
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335134/
https://ncbi.nlm.nih.gov/pubmed/17620712
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910702533X
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