Expression, purification and preliminary X-ray characterization of dl-2-haloacid dehalogenase from Methylobacterium sp. CPA1

dl-2-Haloacid dehalogenase from Methylobacterium sp. CPA1 (dl-DEX Mb) is a unique enzyme that catalyzes the dehalogenation reaction without the formation of an ester intermediate. A recombinant form of dl-DEX Mb has been expressed in Escherichia coli, purified and crystallized using the hanging-drop...

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Detalhes bibliográficos
Main Authors: Omi, Rie, Jitsumori, Keiji, Yamauchi, Takahiro, Ichiyama, Susumu, Kurihara, Tatsuo, Esaki, Nobuyoshi, Kamiya, Nobuo, Hirotsu, Ken, Miyahara, Ikuko
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335131/
https://ncbi.nlm.nih.gov/pubmed/17620717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107027273
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