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Purification, crystallization and preliminary X-ray crystallographic analysis of branched-chain aminotransferase from Deinococcus radiodurans

The branched-chain amino-acid aminotransferase (BCAT), which requires pyridoxal 5′-phosphate (PLP) as a cofactor, is a key enzyme in the biosynthetic pathway of the hydrophobic amino acids leucine, isoleucine and valine. DrBCAT from Deinococcus radiodurans, which has a molecular weight of 40.9 kDa,...

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Detalhes bibliográficos
Main Authors: Chen, Chung-Der, Huang, Tien-Feng, Lin, Chih-Hao, Guan, Hong-Hsiang, Hsieh, Yin-Cheng, Lin, Yi-Hung, Huang, Yen-Chieh, Liu, Ming-Yih, Chang, Wen-Chang, Chen, Chun-Jung
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335077/
https://ncbi.nlm.nih.gov/pubmed/17554170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107020842
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