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Cloning, expression, purification, crystallization and preliminary X-ray analysis of Thermus aquaticus succinyl-CoA synthetase

Succinyl-CoA synthetase (SCS) is an enzyme of the citric acid cycle and is thus found in most species. To date, there are no structures available of SCS from a thermophilic organism. To investigate how the enzyme adapts to higher temperatures, SCS from Thermus aquaticus was cloned, overexpressed, pu...

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Detaylı Bibliyografya
Asıl Yazarlar: Joyce, Michael A., Brownie, Edward R., Hayakawa, Koto, Fraser, Marie E.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: International Union of Crystallography 2007
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2335007/
https://ncbi.nlm.nih.gov/pubmed/17565180
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107017113
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