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Purification, crystallization and preliminary X-ray diffraction studies of a putative UDP-N-acetyl-d-mannosamine dehydrogenase from Pyrococcus horikoshii OT3

A putative UDP-N-acetyl-d-mannosamine dehydrogenase from Pyrococcus horikoshii OT3, an essential enzyme for polysaccharide biosynthesis, has been overexpressed in Escherichia coli and purified. Crystals were obtained using the oil-microbatch method at 291 K. A native data set extending to 1.8 Å reso...

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Detalhes bibliográficos
Main Authors: Lokanath, Neratur K., Pampa, Kudigana J., Kamiya, Toshimi, Kunishima, Naoki
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2334993/
https://ncbi.nlm.nih.gov/pubmed/17565184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107016685
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