Method for the isolation of Escherichia coli K-12 mutants deficient in essential genes.

We developed a general procedure for the induction and identification of mutations in chromosomal essential genes that are located in a diploid region of Escherichia coli K-12. The partial diploidy is conferred by an episome that is temperature sensitive for replication so that a mutant strain will...

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Vydáno v:	J Bacteriol
Hlavní autoři:	Armstrong, K A, Herman, R K
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1976
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC233257/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/770428/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.126.1.38-47.1976
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