Method for the isolation of Escherichia coli K-12 mutants deficient in essential genes.
We developed a general procedure for the induction and identification of mutations in chromosomal essential genes that are located in a diploid region of Escherichia coli K-12. The partial diploidy is conferred by an episome that is temperature sensitive for replication so that a mutant strain will...
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| 發表在: | J Bacteriol |
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| Principais autores: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology (ASM)
1976
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC233257/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/770428/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.126.1.38-47.1976 |
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