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Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes

A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them. Here we present a strategy to overcome this problem, employing a Gal4-inducible luciferase assay to systematically quantify position effects of host chromatin and the...

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Bibliografiset tiedot
Päätekijät: Markstein, Michele, Pitsouli, Chrysoula, Villalta, Christians, Celniker, Susan E, Perrimon, Norbert
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2008
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330261/
https://ncbi.nlm.nih.gov/pubmed/18311141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ng.101
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