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Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes
A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them. Here we present a strategy to overcome this problem, employing a Gal4-inducible luciferase assay to systematically quantify position effects of host chromatin and the...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2008
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2330261/ https://ncbi.nlm.nih.gov/pubmed/18311141 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ng.101 |
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