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An optimized split-ubiquitin cDNA-library screening system to identify novel interactors of the human Frizzled 1 receptor

The yeast split-ubiquitin system has previously been shown to be suitable to detect protein interactions of membrane proteins and of transcription factors in vivo. Therefore, this technology complements the classical split-transcription factor based yeast two-hybrid system (Y2H). Success or failure...

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Autori principali: Dirnberger, Dietmar, Messerschmid, Monika, Baumeister, Ralf
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330230/
https://ncbi.nlm.nih.gov/pubmed/18319286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm1163
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