Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP)

cDNA cloning is a central technology in molecular biology. cDNA sequences are used to determine mRNA transcript structures, including splice junctions, open reading frames (ORFs) and 5′- and 3′-untranslated regions (UTRs). cDNA clones are valuable reagents for functional studies of genes and protein...

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Bibliographic Details
Main Authors: Hoskins, Roger A., Stapleton, Mark, George, Reed A., Yu, Charles, Wan, Kenneth H., Carlson, Joseph W., Celniker, Susan E.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2005
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1301602/
https://ncbi.nlm.nih.gov/pubmed/16326860
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni184
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