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Expression and purification of F-plasmid RepE and preliminary X-ray crystallographic study of its complex with operator DNA

The replication initiator factor RepE of the F plasmid in Escherichia coli is an essential protein that stringently regulates the F-plasmid copy number. The RepE protein has a dual function: its monomer functions as a replication initiator, while its dimer acts as a transcriptional repressor of the...

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Bibliographic Details
Main Authors: Nakamura, Akira, Wada, Chieko, Miki, Kunio
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2007
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330221/
https://ncbi.nlm.nih.gov/pubmed/17401213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107012894
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