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Purification, crystallization and preliminary X-ray diffraction analysis of the non-ATPase subunit Nas6 in complex with the ATPase subunit Rpt3 of the 26S proteasome from Saccharomyces cerevisiae

The non-ATPase subunit Nas6, which is the human orthologue of gankyrin, was co-expressed with the C-terminal domain of the ATPase subunit Rpt3 of the yeast 26S proteasome in Escherichia coli, purified to near-homogeneity and crystallized using the hanging-drop vapour-diffusion method. The protein cr...

תיאור מלא

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מידע ביבליוגרפי
Main Authors: Nakamura, Yoshihiro, Umehara, Takashi, Tanaka, Akiko, Horikoshi, Masami, Padmanabhan, Balasundaram, Yokoyama, Shigeyuki
פורמט: Artigo
שפה:Inglês
יצא לאור: International Union of Crystallography 2007
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2330193/
https://ncbi.nlm.nih.gov/pubmed/17329811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309107004848
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