Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid.
A purified fragment of deoxyribonucleic acid (DNA) that determines resistance to kanamycin and is incapable of self-replication was used to select a self-replicating fragment from an EcoRI endonuclease digest of the sex factor F'lac. This F'lac fragment, exhibiting a molecular weight of 6 X 10(6), c...
Gespeichert in:
| Veröffentlicht in: | J Bacteriol |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
1976
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC233008/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/783124/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.127.2.982-987.1976 |
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